(08 February 2022)
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Archiv: Nature (science media)
Adenovirus vector vaccination reprograms pulmonary fibroblastic niches to support protective inflating memory CD8+ T cells
(15.07.2021)
The ultimate goal of T cell-based vaccination strategies is the induction of long-term immunological protection via effector memory T cells1. Adenovirus (Ad) vector-based vaccines using the backbone of human Ads have been shown in clinical studies
to be highly immunogenic with induction of specific antibody and/or T cell responses to viral or cancer antigens. However, pre-existing anti-Ad antibodies in vaccinated individuals reduces immunogenicity and efficacy of vaccines based on human Ads, which led to the development of non-human vectors based on chimpanzee viruses. Chimeric Ad vectors have been shown to induce long-lived T cell responses against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and to generate polyfunctional central and effector memory T cells against hepatitis C
virus.
(…)
Results
Targeting of human fibroblasts by Ad vectors. Human Ads were first described as cytopathogenic agents isolated from human adenoid tonsils28. Here, we used sliced tissue cultures from human
palatine tonsils to assess the cellular tropism of green fluorescent protein (GFP)-expressing Ad vectors (Extended Data Fig. 1a). Both HuAd5-GFP and chimpanzee (Ch)AdOx1-GFP vectors readily transduced cells in the tissue slides, with PDPN+CD45− cells most
frequently expressing the GFP transgene (Fig. 1a,b). Next, we compared side-by-side the susceptibility to infection with recombinant Ad vectors of short-term cultured tonsillar stromal cells (TSCs) and peripheral blood mononuclear cells (PBMCs). Both ChAdOx1-GFP (Fig. 1c,d) and HuAd5-GFP (Fig. 1e,f ) efficiently transduced TSCs following an incubation of 3 h, while the transduction rate of PBMCs remained low. Even at the high multiplicity of infection (MOI) of 1,000 particles per cells, less than 2% of the hematopoietic cells were GFP-positive.
JAB ‚FOR LIFE‘: AstraZeneca Covid vaccine gives powerful protection that may last a lifetime, study finds
(15.07.2021)
Scientists from Oxford and Switzerland, writing in journal Nature, say T-cell protection is a “key feature” of adenovirus vaccines like the Oxford and J&J jabs.
Researcher Prof Burkhard Ludewig, of Cantonal Hospital in Switzerland, said: „The T-cells that come from these cellular training camps appear to have a very high level of ‘fitness’.
“Adenoviruses have co-evolved with humans over a very long time, and learned a lot about the human immune system in the process.
Die Vertuschungsaktion
(22.12.2020)
Laut Alina Chan, Molekularbiologin am Broad Institute of Harvard und am MIT, kann man die Evolution von SARS-CoV-2 nicht mit der These eines zoonotischen Ursprungs in Einklang bringen, denn das Virus war bereits vollständig für die Mensch-zu-Mensch-Übertragung angepasst, als es zum ersten Mal auftrat.
Die renommierte medizinische Fachzeitschrift „Nature“ erlaubte anscheinend einigen Autoren, ihre Daten heimlich zu ändern, ohne auf diese Korrekturen in den Artikeln hinzuweisen.
Chans Untersuchungen zeigen, dass die Autoren ihre Proben umbenannt, falsch zugeordnet und ein Genomprofil generiert haben, das mit keiner ihrer Proben übereinstimmt. In anderen Papers fehlen Daten.
Das Coronavirus RaTG13, zu 96 Prozent mit SARS-CoV-2 identisch und somit der engste Verwandte, ist in Wirklichkeit btCoV-4991. Dieses Genom wurde schon 2013 in Proben nachgewiesen und 2016 veröffentlicht.
Did Top Medical Journal Help Cover Up Origins of SARS-CoV-2?
(11.09.2020)
– According to Alina Chan, a molecular biologist at the Broad Institute of Harvard and MIT, SARS-CoV-2 did not evolve in a manner you’d expect, had it jumped from an animal to a human. It sprang into action fully evolved for human transmission
– It appears Nature, a top medical journal, has allowed authors to secretly alter data sets in their papers without publishing notices of correction
– Chan’s investigation reveals authors have renamed samples, failed to attribute them properly, and produced a genomic profile that doesn’t match the samples in their paper. Others are missing data
– RaTG13 — the coronavirus that most resembles SARS-CoV-2, being 96% identical — is actually btCoV-4991, a virus found in samples collected in 2013 and published in 2016
– If SARS-CoV-2, the virus responsible for COVID-19 and the subsequent response to it, came from a lab, then we need to reassess the future of gain-of-function research that allows for the weaponization of viruses
Get ready. This is going to be an important thread. Election season will be over soon and hopefully more people will devote some attention to this… I‘m going to walk through a timeline of SARS2-related virus data published in the months after the outbreak. (1/30)
(25 Oct 20)
Since the outbreak in late 2019, events have been unfolding at such a fast pace that it is difficult to keep track of what happened and in what order.
I use visualizations of the timeline to follow key events relating to the search for the animal host of SARS2. (2/30)
Even today, I still hear people saying that SARS-CoV-2 came from pangolins and a Seafood market in Wuhan. I hope this analysis will help to clear things up. It will refresh us on significant early pandemic events and major publications discussing the origins of the virus (3/30).
Detection of phosphine: new aspects of the phosphorus cycle in the hydrosphere
(26.05.1988)
According to our measurements and calculations, about 5g of phosphorus per day was released as phosphine from an Imhoff tank settling 2,000 m3 per day of raw sewage. Under laboratory conditions, it was also demonstrated that phosphine is released by bacterial reduction from a medium containing inorganic phosphorus.
Phosphine gas in the cloud decks of Venus
(14.09.2020)
Here we report the apparent presence of phosphine (PH3) gas in Venus’s atmosphere, where any phosphorus should be in oxidized forms. Single-line millimetre-waveband spectral detections (quality up to ~15σ) from the JCMT and ALMA telescopes have no other plausible identification.
A major problem with the Resplandy et al. ocean heat uptake paper
(6.11.2018) I wanted to make sure that I had not overlooked something in my calculations, so later on November 1st I emailed Laure Resplandy querying the ΔAPOClimate trend figure in her paper and asking for her to look into the difference in our trend estimates as a matter of urgency, explaining that in view of the media coverage of the paper I was contemplating web-publishing a comment on it within a matter of days. To date I have had no substantive response from her, despite subsequently sending a further email containing the key analysis sections from a draft of this article.
How might Laure Resplandy [xiv] have miscalculated the ΔAPOClimate trend as 1.16 per meg per year?
Global Warming: Another Doomsday Climate Model Flunks A Math Test
(15.11.2018) Media around the world seized upon the report as yet another indicator of climate-change doom and runaway global warming. No surprise, since most of the media faithfully adhere to the Holy Church of Global Warming.