Archiv: Victor M. Corman


13:02 [ CormanDrostenReview.com ]

Review report Corman-Drosten et al. Eurosurveillance 2020

(27.11.2020)

It should be noted that there is no mention anywhere in the Corman-Drosten paper of a test being positive or negative, or indeed what defines a positive or negative result. These types of virological diagnostic tests must be based on a SOP, including a validated and fixed number of PCR cycles (Ct value) after which a sample is deemed positive or negative. The maximum reasonably reliable Ct value is 30 cycles. Above a Ct of 35 cycles, rapidly increasing numbers of false positives must be expected .

PCR data evaluated as positive after a Ct value of 35 cycles are completely unreliable.

12:57 [ Weltgesundheitsorganisation - WHO.int ]

Berlin, Jan 17th, 2020: Diagnostic detection of 2019-nCoV by real-time RT-PCR

All oligonucleotides were synthesised and provided by Tib-Molbiol, Berlin. Thermal cycling was performed at 55°C for 10 min for reverse transcription, followed by 95°C for 3 min and then 45 cycles of 95°C for 15 s, 58°C for 30 s.

22.01.2021 - 18:52 [ Weltgesundheitsorganisation - WHO.int ]

Berlin, Jan 17th, 2020: Diagnostic detection of 2019-nCoV by real-time RT-PCR

All oligonucleotides were synthesised and provided by Tib-Molbiol, Berlin. Thermal cycling was performed at 55°C for 10 min for reverse transcription, followed by 95°C for 3 min and then 45 cycles of 95°C for 15 s, 58°C for 30 s.

13.12.2020 - 14:55 [ CormanDrostenReview.com ]

Review report Corman-Drosten et al. Eurosurveillance 2020

(27.11.2020)

It should be noted that there is no mention anywhere in the Corman-Drosten paper of a test being positive or negative, or indeed what defines a positive or negative result. These types of virological diagnostic tests must be based on a SOP, including a validated and fixed number of PCR cycles (Ct value) after which a sample is deemed positive or negative. The maximum reasonably reliable Ct value is 30 cycles. Above a Ct of 35 cycles, rapidly increasing numbers of false positives must be expected .

PCR data evaluated as positive after a Ct value of 35 cycles are completely unreliable.

13.12.2020 - 14:50 [ National Center for Biotechnology Information - ncbi.nlm.nih.gov ]

Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

(23.01.2020)

All oligonucleotides were synthesised and provided by Tib-Molbiol (Berlin, Germany). Thermal cycling was performed at 55 °C for 10 min for reverse transcription, followed by 95 °C for 3 min and then 45 cycles of 95 °C for 15 s, 58 °C for 30 s.

(…)

This work was funded by European Union DG Research through projects Prepare (GA602525), Compare (GA643476), and EVAg (GA653316); by European Union DG SANCO through EVD-LabNet, as well as by the German Ministry of Research through projects RAPID (01KI1723A) and DZIF (301-4-7-01.703).

13.12.2020 - 14:41 [ WHO.int ]

Berlin, Jan 17th, 2020: Diagnostic detection of 2019-nCoV by real-time RT-PCR

All oligonucleotides were synthesised and provided by Tib-Molbiol, Berlin. Thermal cycling was performed at 55°C for 10 min for reverse transcription, followed by 95°C for 3 min and then 45 cycles of 95°C for 15 s, 58°C for 30 s.