13.12.2020 - 14:50 [ National Center for Biotechnology Information - ncbi.nlm.nih.gov ]

Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

(23.01.2020)

All oligonucleotides were synthesised and provided by Tib-Molbiol (Berlin, Germany). Thermal cycling was performed at 55 °C for 10 min for reverse transcription, followed by 95 °C for 3 min and then 45 cycles of 95 °C for 15 s, 58 °C for 30 s.

(…)

This work was funded by European Union DG Research through projects Prepare (GA602525), Compare (GA643476), and EVAg (GA653316); by European Union DG SANCO through EVD-LabNet, as well as by the German Ministry of Research through projects RAPID (01KI1723A) and DZIF (301-4-7-01.703).