All oligonucleotides were synthesised and provided by Tib-Molbiol, Berlin. Thermal cycling was performed at 55°C for 10 min for reverse transcription, followed by 95°C for 3 min and then 45 cycles of 95°C for 15 s, 58°C for 30 s.
All oligonucleotides were synthesised and provided by Tib-Molbiol, Berlin. Thermal cycling was performed at 55°C for 10 min for reverse transcription, followed by 95°C for 3 min and then 45 cycles of 95°C for 15 s, 58°C for 30 s.